In Vitro Antibacterial Effects of the Chimeric Peptides from Chicken and Pig Antimicrobial Peptide NK-Lysin

Antimicrobial peptides (AMPs) play an important role in innate immunity against pathogenic infections. AMPs exterminate bacteria by disrupting cell membranes or inhibiting intracellular molecules. NK-2, first identified pigs and derived from NK-lysin, has antimicrobial effects parasites. In this study, chimeric (cpNK) of chicken pig NK-2 cpNK-derived (cpNK-a1 cpNK-a2) were synthesized, their various such as Escherichia coli, Salmonella spp., Listeria monocytogenes, Staphylococcus aureus, methicillin-resistant aureus (MRSA) investigated. The structure cpNK, include α-helix like peptide net charge was +9 porcine NK-2. cpNK showed powerful bactericidal most bacterial species, including MRSA, especially gram-negative bacteria. Furthermore, short peptides, cpNK-a1 a2 also activity, but the weaker than those cpNK. Therefore, we conclude that cpNK- have potential to be used antibiotic alternatives.